Journal of Pharmacological Sciences | |
Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells | |
Susumu Ohya1  Yuji Imaizumi1  Daiju Yamazaki1  Kiyofumi Asai2  | |
[1] Department of Molecular & Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan;Department of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, Japan | |
关键词: brain capillary endothelial cell; store-operated channel; receptor-operated channel; | |
DOI : 10.1254/jphs.SC0070080 | |
学科分类:药学 | |
来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society | |
【 摘 要 】
References(15)Cited-By(7)ATP-receptor (P2Y) stimulation induced sustained Ca2+-entry, which was essential for the enhanced cell-proliferation in t-BBEC117, an immortalized cell-line derived from bovine brain endothelial cells. Application of Ca2+ following store-depletion with thapsigargin in Ca2+-free solution induced Ca2+-entry through store-operated channels (SOCs). Ca2+-entry induced by ATP or 1-oleoyl-2-acetyl-sn-glycerol (OAG) together with Ca2+ was significantly larger than that by Ca2+ alone, suggesting the involvement of receptor-operated channels (ROCs) in the Ca2+-entry. Results obtained using pharmacological tools suggest that the contribution of Ca2+ sources to ATP-induced [Ca2+]i rise in t-BBEC117 is estimated as approximately 2:1:2 for Ca2+-release and Ca2+-entry though SOCs and ROCs, respectively.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201911300448391ZK.pdf | 569KB | download |