Characteristics of the ATP-Induced Ca2+-Entry Pathway in the t-BBEC 117 Cell Line Derived From Bovine Brain Endothelial Cells

Journal of Pharmacological Sciences

Susumu Ohya¹  Yuji Imaizumi¹  Daiju Yamazaki¹  Kiyofumi Asai² 

[1] Department of Molecular & Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Japan;Department of Molecular Neurobiology, Graduate School of Medical Sciences, Nagoya City University, Japan

关键词: brain capillary endothelial cell;    store-operated channel;    receptor-operated channel;   

DOI  :  10.1254/jphs.SC0070080

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(7)ATP-receptor (P2Y) stimulation induced sustained Ca2+-entry, which was essential for the enhanced cell-proliferation in t-BBEC117, an immortalized cell-line derived from bovine brain endothelial cells. Application of Ca2+ following store-depletion with thapsigargin in Ca2+-free solution induced Ca2+-entry through store-operated channels (SOCs). Ca2+-entry induced by ATP or 1-oleoyl-2-acetyl-sn-glycerol (OAG) together with Ca2+ was significantly larger than that by Ca2+ alone, suggesting the involvement of receptor-operated channels (ROCs) in the Ca2+-entry. Results obtained using pharmacological tools suggest that the contribution of Ca2+ sources to ATP-induced [Ca2+]i rise in t-BBEC117 is estimated as approximately 2:1:2 for Ca2+-release and Ca2+-entry though SOCs and ROCs, respectively.

【 授权许可】

Unknown