コイルドコイル中の柔軟領域の予測

Chem-Bio Informatics Journal

美宅 成樹³  谷澤 英樹³  ギミ�? ガンガ�??D.¹  谷口 美恵子² 

[1] Research Centre Juelich, Institute of Neurosciences and Biophysics, INB-2, Molecular Biophysics;名古屋大学大学院工学研究科化学生物分野;名古屋大学大学院工学研究科応用物理学分野

关键词: coiled coil;    Coiled coil;    SOSUIcoil;    fragile point;    AFM;    atomic force microscopy;   

DOI  :  10.1273/cbij.9.12

学科分类：生物化学/生物物理

来源: Chem-Bio Informatics Society

【 摘 要 】

References(30)Cited-By(1)A prediction system for identifying the region of flexible regions of the coiled coil was developed to determine the bending positions of the myosin rods using atomic force microscopy (AFM) and to analyze the molecular structures of proteins containing coiled coils. The prediction system comprises two modules: identification of heptad break points and prediction of fragile points in the coiled coil due to the hydrophilic core or hydrophobic outfield region. Here, we investigated the myosin rods using this prediction system. The results of AFM imaging showed four main flexible regions in a single myosin rod and of the 17 possible fragile points predicted, 16 were located in the four experimental bending regions. Next, we examined the enhanced fluctuation around these predicted fragile points using the B-factor for the three dimensional structure of coiled coil proteins from the SCOP database and found that the fluctuations in the hydrophilic core regions were significantly larger than those in the regions of the normal coiled coil. In contrast, the fluctuations in the hydrophobic outfield regions were reduced, suggesting a structural change of the coiled coils to balance these regions. Thus, the dynamic changes in the structure of the coiled coils around the fragile points may be related to the biological functions of the proteins. The prediction tool which developed in this work was incorporated in the SOSUIcoil system which predicts the coiled coil regions.

【 授权许可】

Unknown