Cooling of the canine epididymis at 4ºC and recovery of epididymal sperm using ACP-106c

Pesquisa Veterinária Brasileira

Araújo, Airton A.²  Silva, Herlon V.R.¹  Freitas, Luana A.¹  Mota Filho, Antonio C.¹  Silva, Lúcia D.M.¹  Nunes, Thalles G.P.¹ 

[1] Universidade Estadual do Ceará, Fortaleza, Brasil;UECE, Fortaleza

关键词: Refrigeration;    epididymis;    dogs.;   

DOI  :  10.1590/S0100-736X2013000900018

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

The study aimed to assess the quality of sperm recovered from the epididymal cauda after cooling the complex testis-epididymis (CTE) of dogs using ACP-106c extender. Sixty adult male dogs, weighing 10-20 kg were used. After euthanasia, CTE was removed and it was immersed in 0.9% saline and transported to the laboratory in cooler at 30ºC. For cooling and recovery of epididymal spermatozoa, the 60 pairs of CTE were divided into four groups, according to the refrigeration time of the CTE and subsequent sperm recovery: G0h, G6h, G12h and G18h, wherein each pair of CTE remained zero, six, 12 or 18 hours at 4ºC respectively. The recovery of sperm epididymal tail was conducted by flotation technique using ACP-106c or Tris extender. For each epididymis, it was added 1.0 mL of either extenders, preheated to 37ºC for 5 minutes. They were then centrifuged at 800g/5 minutes to remove the cell debris. Morphology, functionality and total and progressive motility, and parameters obtained by CASA were evaluated. Data were analyzed by ANOVA followed by Tur

【 授权许可】

CC BY-NC