Fingerprinting of Flavobacterium psychrophilum isolates by ribotyping and plasmid profiling

Diseases of Aquatic Organisms

Maria C. Urdaci¹  Chehid Chakroun¹  Francine Grimont¹  Jean-François Bernardet¹ 

关键词: Flavobacterium psychrophilum;    Cold-water disease;    Rainbow trout fry syndrome;    Ribotyping;    Plasmid profiling;    Epidemiology;   

DOI  :  10.3354/dao033167

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Flavobacterium psychrophilum is the agent of cold-water disease and rainbow trout fry syndrome in salmonid fish worldwide. Ribosomal RNA gene restriction patterns (ribotypes) and plasmid profiles were determined on a collection of85 strains isolated from different countries and fish species. Several ribotypes were obtained by using the restriction endonucleases HincII and PvuII. Computer analysis of the ribotypes revealed that some of them were clearly associatedwith the fish species from which the strains were isolated, whereas no correlation with the geographical origin was found. Most of the strains harboured at least one plasmid and several different plasmid profiles were observed, even among strains sharingthe same ribotype. These methods, used alone or in combination with other typing techniques, can be considered powerful tools for the epidemiological tracing of F. psychrophilum infections.

【 授权许可】

Unknown