Parathyroid Hormone-Related Protein (PTHrP) Produced by Dog Lymphoma Cells

Journal of Veterinary Medical Science

Atsuhiko HASEGAWA²  Hajime TSUJIMOTO¹  Masaharu HISASUE¹  Takuya MIZUNO¹  Toshihiro WATARI⁴  Peter F. MOORE³  Akiko KUBOTA²  Rui KANO² 

[1] Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Toky;Department of Pathobiology, Nihon University School of Veterinary Medicine;Department of Veterinary Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California at Davis;Laboratory of Comprehensive Veterinary Clinical Studies, Nihon University School of Veterinary Medicine

关键词: hypercalcemia;    lymphoma cell;    mRNA;    parathyroid hormone-related protein;    RT-PCR;   

DOI  :  10.1292/jvms.64.835

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(11)Cited-By(4)Parathyroid hormone-related protein (PTHrP) was investigated in a canine lymphoma case with hypercalcemia by means of immunoradiomentric assay (IRMA) and molecular analysis. The plasma calcium level of the patient dog was 13.7 mg/dl. The PTHrP concentration examined by IRMA was 6.1 pmol/L in the plasma sample from the dog, but it was undetectable (< 1.1 pmol/L) in plasma samples from 4 lymphoma cases without hypercalcemia or 5 normal dogs. The PTHrP concentration examined in the culture supernatant of the lymphoma cells from this case was 1.3 pmol/L, whereas those of the lymphoma cells from a lymphoma case without hypercalcemia was undetectable. PTHrP mRNA was clearly detected not only in the lymphoma cells from this dog with hypercalcemia but also in lymphoma cells from 4 lymphoma cases without hypercalcemia and 2 canine lymphoma cell lines.

【 授权许可】

Unknown