Effect of acute heat stress on hepatic and cerebral messenger RNA heat shock protein 70 and heat shock protein 70 level of broiler chicks from 2 to 5 days old of different strains

Revista Brasileira de Zootecnia

Rutz, Fernando¹  Dionello, Nelson José Laurino³  Ferro, Jesus Aparecido⁴  Macari, Marcos⁵  Ferro, Maria Inês Tiraboschi⁴  Furlan, Luiz Roberto² 

[1] Universidade Federal de Pelotas, Pelotas, Brasil;UNESP, Botucatu;UFPel;UNESP;Universidade Estadual Paulista, Jaboticabal, Brasil

关键词: acute heat stress;    hepatic and brain Hsp70;    protein and mRNA Hsp70;    Northern Blotting and Western Blotting     IntroduçãoA existência de uma base genética para resistência ao calor foi comprovada por EBERHART e WASHBURN (1993b);    os quais concluíram que a criação de aves com maior resistência ao calor seria a alternativa para as regiões de climas tropicais;   

DOI  :  10.1590/S1516-35982001000600018

学科分类：兽医学

来源: Universidade Federal de Vicosa

【 摘 要 】

- Eighty broiler chicks, Naked neck (Na/na) and Hubbard-Pettersen strains, were exposed to acute heat stress (36-37°C), from 2 to 5 days of age, and body weight and cloacal temperature were measured in the beginning and at the end of the heat stress period (5 h). Birds were sacrificed at the end and liver and brain samples were collected and analyzed using Western Blotting and Northern Blotting to quantify Hsp70 levels and mRNA Hsp70 transcript, respectively. The brain tissue had higher Hsp70 level than liver tissue. Before heat stress it was not observed difference in the Hsp70 expression between strains. After heat stress in the liver it was observed in increase in the Hsp70 level (naked neck birds at 4 and 5 days), and in the brain a reduced Hsp70 level was found when compared with the control birds (Naked neck birds at 2 days). The size of the mRNA Hsp70 transcript was 2.7 kb. The data of this experiment suggest that to the brain tissue heat stress affect Hsp70 at transcriptional and translational levels, and for hepatic tissue the effect is only at translational level, for different lines.

【 授权许可】

CC BY-NC