Experimental neuropathogenesis of bovine herpesvirus 5 infection in rabbits

Pesquisa Veterinária Brasileira

Vogel, Fernanda Silveira Flores¹  Weiblen, Rudi¹  Roehe, Paulo Michel³  Flores, Eduardo Furtado¹  Spilki, Fernando Rosado²  Almeida, Sabrina Ribeiro de⁴  Dezengrini, Renata¹ 

[1] UFSM, Santa Maria, Brasil;Centro Universitário Feevale, Novo Hamburgo, Brasil;Fepagro Saúde Animal, Eldorado do Sul, Brasil;Centro de Pesquisas Aggeu Magalhães, Recife, Brasil

关键词: Bovine herpesvirus 5;    BoHV-5;    rabbits;    experimental model.;   

DOI  :  10.1590/S0100-736X2009000100001

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

Several aspects of the biology of bovine herpesvirus 5 (BoHV-5) have been studied in rabbits, which develop acute infection and neurological disease upon experimental inoculation. The acute infection is followed by the establishment of latent infection, which can be naturally or artificially reactivated. The first experiments in rabbits established a protocol for virus inoculation and monitoring the infection, and characterized the main virological, clinical and pathological aspects of the acute infection. The pathogenesis of acute infection, from the initial viral replication at site of inoculation, pathways and kinetics of viral transport to the brain, distribution and virus replication in the central nervous system (CNS), cellular and tissue tropism, clinical signs and CNS pathology have been extensively studied using this animal model. Subsequently, several biological and molecular aspects of latent BoHV-5 infection have also been elucidated upon inoculation of rabbits. Rabbits have also been used to investigate the phenotype (neuroinvasiveness, neurogrowth) of field isolates and recombinant vaccine candidates, protection by passive immunity, vaccine protection, the efficacy of anti-viral drugs and support therapies for neurological disease. This animal model was also used to investigate the origin and distribution of electric impulses involved in seizures - a hallmark of BoHV-5 induced neurological infection - and also to test the efficacy of anti-convulsivants. In spite of the possible differences between rabbits and cattle - the natural host of the virus - the observations taken from this experimental model have greatly contributed to the knowledge of the biology of BoHV-5 infection. The present article presents a review of the main published and unpublished results and observations by our group, comprising more than a decade of studies on the pathogenesis of BoHV-5 infection in the rabbit model. Index

【 授权许可】

CC BY-NC