Natural and experimental infection of white spot syndrome virus (WSSV) in benthic larvae of mud crab Scylla serrata

Diseases of Aquatic Organisms

Chu-Fang Lo¹  Ya-Lin Chiu¹  Chen-Fang Chang¹  Guang-Hsiung Kou¹  Li-Li Chen¹ 

关键词: Natural infection;    WSSV;    Benthic larvae;    Crab seeds;    Scylla serrata;    Tissue tropism;    PCR;   

DOI  :  10.3354/dao040157

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: White spot syndrome virus (WSSV), the causative agent of white spot syndrome in shrimp, has a wide host range which extends to crabs, copepods and other arthropods. In this study, benthic larvae of the mud crab Scylla serrata werecaptured from Taiwan¹s coastal waters and screened for the presence of WSSV by polymerase chain reaction (PCR) and in situ hybridization. WSSV was detected in around 60% of the larvae, and this prevalence rate remained fairly constant when thecaptured larvae were subsequently maintained in an aerated system in the laboratory. WSSV-free larvae obtained from a hatchery were challenged by immersion in a WSSV inoculum. Fifteen days after challenge, cumulative mortality in the experimental groupreached 43% compared to 20% in the control group. PCR detection of WSSV in both moribund and surviving specimens clearly implicated the virus as the cause of death in most cases. Histological and in situ hybridization data confirmed that WSSVtissue tropism in Scylla serrata crab larvae is similar to that found in shrimp.

【 授权许可】

Unknown