A Direct Enzyme Immunoassay for the Measurement of Furosemide in Horse Plasma

Journal of Veterinary Medical Science

Shun-ichi NAGATA¹  Masao KUWAJIMA¹  Masahiko KUROSAWA¹ 

[1] Laboratory of Racing Chemistry

关键词: enzyme immunoassay;    equine;    furosemide;   

DOI  :  10.1292/jvms.69.305

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(1)A new enzyme immunoassay (EIA) for the measurement of furosemide in horse plasma is described. The lower limit of detection of this EIA method was 7.8 ng/ml. The intra-and inter-assay coefficients of variation ranged from 2.5% to 4.9% and 7.5% to 9.8%, respectively. Cross-reactivity with other compounds was not observed. There was a high correlation (r2=0.987) between the high-performance liquid chromatography and EIA results obtained for furosemide concentrations in horse plasma. These results indicate that the newly developed EIA method is useful for the quantitative analysis of furosemide in horse plasma.

【 授权许可】

Unknown