Polymerase Chain Reaction (PCR) for the Detection of Herpesvirus in Tortoises

Journal of Veterinary Medical Science

Hideo FUJITANI²  Yasuo NOMURA¹  Kayoko UEMURA¹  Toru ISHIBASHI³  Yumi UNE¹  Masaru MURAKAMI² 

[1]Department of Veterinary Pathology

[2]Department of Molecular Biology

[3]Animal Hospital, School of Veterinary Medicine, Azabu University

关键词: Alphaherpesvirus;    PCR;    tortoise;   

DOI  :  10.1292/jvms.62.905

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(14)Cited-By(11)A polymerase chain reaction (PCR)-based method using a herpesvirus consensus primer was assessed for the identification of herpesviral infections in tortoises. A single band of about 230 bp was detected in PCR products from two out of twenty swabs taken from the oral cavity, three out of three paraffin-embedded tissue sections from the liver (two cases) and oral mucosa (one case), and one out of two fresh tissue samples from the oral mucosa. Nucleotide sequencing of these PCR products indicated that the herpesvirus present in these tortoises might belong to the alphaherpesvirinae. PCR using swabs and biopsy tissues was a sensitive and highly specific method for the diagnosis of herpesviral infections in tortoises.

【 授权许可】

Unknown