Early-Onset Podocyte Injury and Glomerular Sclerosis in Osborne-Mendel Rats

Journal of Veterinary Medical Science

Makoto ISHIKAWA²  Shoko ISHIHARA²  Kazuya KAWANO¹  Ryosuke KOBAYASHI²  Takashi KATO²  Kyohei YASUNO²  Rio SAITO²  Kikumi OGIHARA⁴  Junichi KAMIIE³  Kinji SHIROTA²  Tomoshige BABA² 

[1] Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd.;Research Institute of Biosciences, Azabu University;Laboratory of Veterinary Pathology, School of Veterinary Medicine, Azabu University;Laboratory of Environmental Pathology, School of Environmental Health, Azabu University

关键词: glomerulonephropathy;    kidney;    podocyte;    proteinuria;    rat;   

DOI  :  10.1292/jvms.10-0094

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(26)Cited-By(7)Progressive glomerular injury associated with early-onset proteinuria was investigated in male Osborne-Mendel (OM) rats aged 5 to 20 weeks. Age-matched male Fischer 344 (F344) rats were used for comparison. OM rats developed mild hypertension and selective proteinuria (albuminuria) from 5 weeks of age, and non-selective proteinuria from 7 weeks of age. Light microscopy of OM kidney revealed hyaline droplets in the podocyte at 5 weeks of age and vacuolation of podocytes and adhesion of the capillary loop to the Bowman's capsule at 7 weeks of age. Segmental glomerulosclerosis developed in OM rats from 15 weeks of age, and global sclerosis appeared at 20 weeks of age. Desmin, a marker of podocye injury, was expressed in podocytes from 10 weeks of age, and the intensity of expression increased with age. Ultrastructurally, damage to podocytes such as effacement of foot processes, decreasing number of filtration slits, and rearrangement of the actin cytoskeleton were observed from 5 weeks of age in OM rat. Glomerular volume in OM rats increased with age and was consistently higher than in age-matched F344 rats. The number of WT-1-positive podocytes and vimentin-positive podocyte area were lower in OM rats and decreased with age. These findings suggest that glomerulonephropathy in male OM rats is associated with glomerular hypertrophy, progressive podocytopathy, and a reduction in podocyte number and area. Renal injury in OM rats was associated with development of early-onset proteinuria and was more progressive than in age-matched F344 rats.

【 授权许可】

Unknown