Preliminary phytochemical analysis and antioxidant, antiinflammatory and antiproliferative activities of the bark ethanol extracts of Zanthoxylum fagara (L.) Sarg. (Rutaceae)

Revista Cubana de Plantas Medicinales

Coy Barrera, Ericsson David²  Cuca Suárez, Luís Enrique¹  Macías Villamizar, Víctor Enrique³ 

[1] Grupo de Productos Naturales Vegetales, Bogotá, Colombia;Facultad de Ciencias Básicas, Cundinamarca, Colombia;Grupo de Fitoquímica, Santa Marta, Magdalena, Colombia

关键词: análisis fitoquímico;    actividad antioxidante;    actividad antiproliferativa;    actividad antiinflamatoria;    Zanthoxylum;    Rutaceae;   

DOI  :  

学科分类：药理学

来源: Editorial Ciencias Medicas

【 摘 要 】

Introduction: the Z. fagara species having multiple ethnobotanical uses, and a variety of secondary metabolites becomes a promising object of study at phytochemical and/or pharmacological settings. Objectives: to detect the possible presence of certain groups of secondary metabolites by means of a preliminary phytochemical analysis and to evaluate the antioxidant, anti-inflammatory and antiproliferative activities of the ethanolic extract from Zanthoxylum fagara (L.) Sarg. (Rutaceae)bark. Methods: the bark extract underwent preliminary phytochemical analysis using tests to evaluate antioxidant action (DPPH, TBARS test and FeSO4-induced peroxidation), anti-inflammatory activity (using mouse ear edema induced by acetate 12-O-tetradecanoylphorbol, TPA) and antiproliferative property (in treating tumor cells, sulphorodamine B protein (SRB) in microculture assay was used in addition to measuring cell viability and growth). Results: alkaloids, flavonoids, tannins and terpenes were found in the extract. The antioxidant activity at 10, 100 and 1000 ppm concentrations, exhibited inhibition percentages of 14.9; 84.5 and 92.5 % respectively [compared to DPPH]; 92.8 % (1 000 ppm) [TBARS test], and 4.2; 47.1; and 93.9 %, respectively, in the FeSO4-induced peroxidation. The anti-inflammatory activity showed inhibition percentage of 45.3 %, and in the case of anti-proliferation, the extract was moderately active against cancer cell lines K562, being the inhibition percentage equals to 55.5 %. In the other tested lines (U251, PC-3, HCT-15, MCF-7, and Skule) the inhibition percentage was lower than 50 %. Conclusions: the preliminary phytochemical testing showed the presence of alkaloids, flavonoids, tannins and terpenes. Antioxidant and anti-inflammatory properties were observed at above 100 ppm extract concentrations, whereas the antiproliferative activity was moderately effective against leukemia cancer cell lines K562.

【 授权许可】

CC BY-NC