Journal of Veterinary Medical Science | |
Enrichment for Isolating Salmonella Choleraesuis and other Salmonella spp. from Pigs | |
Shizuo SATO1  Koshi YAMAMOTO2  Takayuki OSUMI1  Tetsuo ASAI1  Takanori NAMIMATSU1  | |
[1] Zen-noh Institute of Animal Health;National Institute of Animal Health | |
关键词: enrichment; Salmonella; swine; | |
DOI : 10.1292/jvms.65.949 | |
学科分类:兽医学 | |
来源: Japanese Society of Veterinary Science | |
【 摘 要 】
References(20)The growth of Salmonella Choleraesuis was examined in Rappaport Vassiliadis broth (RV) and Hajna-tetrathionate broth (HTT) at 37 and 42°C. As the enrichment in RV at 37°C was satisfactory for isolating S. Choleraesuis, we used this enrichment for isolation from the samples collected from 15 asymptomatic pigs reared on a S. Choleraesuis contaminated farm. S. Choleraesuis was frequently isolated from six pigs (40.0%) under field conditions. The isolation of other Salmonella serovars than S. Choleraesuis was attempted by using both RV enrichment at 37°C and HTT enrichment at 42°C. Salmonella organisms were isolated from 156 (44.8%) of 348 fecal samples and more frequently with HTT at 42°C (43.4%) than with RV at 37°C (20.9%). If other serovars in addition to S. Choleraesuis are to be surveyed, HTT enrichment should be used in combination with RV enrichment.
【 授权许可】
Unknown
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RO201911300411573ZK.pdf | 127KB | download |