Enrichment for Isolating Salmonella Choleraesuis and other Salmonella spp. from Pigs

Journal of Veterinary Medical Science

Shizuo SATO¹  Koshi YAMAMOTO²  Takayuki OSUMI¹  Tetsuo ASAI¹  Takanori NAMIMATSU¹ 

[1] Zen-noh Institute of Animal Health;National Institute of Animal Health

关键词: enrichment;    Salmonella;    swine;   

DOI  :  10.1292/jvms.65.949

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)The growth of Salmonella Choleraesuis was examined in Rappaport Vassiliadis broth (RV) and Hajna-tetrathionate broth (HTT) at 37 and 42°C. As the enrichment in RV at 37°C was satisfactory for isolating S. Choleraesuis, we used this enrichment for isolation from the samples collected from 15 asymptomatic pigs reared on a S. Choleraesuis contaminated farm. S. Choleraesuis was frequently isolated from six pigs (40.0%) under field conditions. The isolation of other Salmonella serovars than S. Choleraesuis was attempted by using both RV enrichment at 37°C and HTT enrichment at 42°C. Salmonella organisms were isolated from 156 (44.8%) of 348 fecal samples and more frequently with HTT at 42°C (43.4%) than with RV at 37°C (20.9%). If other serovars in addition to S. Choleraesuis are to be surveyed, HTT enrichment should be used in combination with RV enrichment.

【 授权许可】

Unknown