Responses of cloned rainbow trout Oncorhynchus mykiss to an attenuated strain of infectious hematopoietic necrosis virus

Diseases of Aquatic Organisms

Scott E. LaPatra¹  Richard Dixon¹  William D. Shewmaker¹  Christine R. Pedrow¹  Jeong-Woo Park¹  Gary H. Thorgaard¹  Sandra S. Ristow¹ 

关键词: IHNV;    Vaccine;    Homozygous clones;    Rainbow trout;    Immunity;   

DOI  :  10.3354/dao042163

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The objective of this work was to examine the response of homozygous clones of rainbow trout to vaccination by an attenuated strain (Nan Scott Lake; NSL) of infectious hematopoietic necrosis virus (IHNV). Adult rainbow trout of the Hot CreekStrain (YY males maintained in a recirculating system at 12°C) were injected 3 times with 10⁵ to 10⁷ plaque forming units (pfu) of NSL. Intraperitoneal injections were given at Day 0 and at 2 and 4 mo post-infection. All fish werenon-lethally bled at monthly intervals for 18 mo. Serum from each fish was analyzed by the complement-dependent neutralization assay and by western blot against purified NSL virus. The highest virus neutralization titers were detected 4 mo after the firstinjection, and peaked at 1280. When sera were analyzed by western blot, the predominating responses of the serum from immunized fish on the reduced western blot were against M1, a matrix protein of the virus and to a 90 kDa stress protein. The 90 kDaprotein was identified by a monoclonal antibody as a stress protein derived from the CHSE-214 cells in which the purified IHN virus was grown and which associates with the virus during purification.

【 授权许可】

Unknown