Rainbow trout Oncorhynchus mykiss ladderlectin, but not intelectin, binds viral hemorrhagic septicemia virus IVb

Diseases of Aquatic Organisms

K. M. Young¹  A. Reid¹  J. S. Lumsden¹ 

关键词: Oncorhychus mykiss;    Pattern recognition receptor;    Viral hemorrhagic septicemia virus;    Innate immunity;    Plasma proteins;    Binding;    Ladderlectin;    Intelectin;   

DOI  :  10.3354/dao02358

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The innate immune system of fish is critical for rapid detection and immediate response to infection, as well as to orchestrate the adaptive branch of the immune system. Rainbow trout Oncorhynchus mykiss ladderlectin and intelectin are plasma pattern recognition receptors (PRR) for bacterial and fungal pathogens of rainbow trout, but their role as PRRs for virus is unknown. Viral hemorrhagic septicemia virus (VHSV) IVb is a recently described fish pathogen in the Great Lakes, and rainbow trout can be experimentally infected. Using an indirect enzyme-linked immuno­sorbent assay, rainbow trout plasma ladderlectin significantly (p < 0.05) bound purified VHSV while ­int­electin did not. In addition, plasma ladderlectin but not intelectin was eluted from a VHSV-conjugated Toyopearl column using EDTA. Protein identification was confirmed with polyclonal antiserum used with slot immunoblot, 1-dimensional sodium dodecyl sulphate polyacrylamide electrophoresis, and Western immunoblot.

【 授权许可】

Unknown