Isolation of a subtilisin-like serine protease gene (MyxSubtSP) from spores of Myxobolus cerebralis, the causative agent of whirling disease

Diseases of Aquatic Organisms

Christoph Dörfler¹  Mansour El-Matbouli¹ 

关键词: Salmonid whirling disease;    Myxobolus cerebralis;    Serine protease;    Cloning;   

DOI  :  10.3354/dao073245

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Proteases play important roles in parasite life cycles and host–parasite interactions. They are pathogenesis factors of many pathogenic organisms and are hence potential targets for chemotherapeutic treatment of disease. We identified a subtilisin-like serine protease gene, MyxSubtSP, expressed by Myxobolus cerebralis. After PCR with subtilisin-like serine protease primers, the gene was cloned, sequenced and aligned against the NCBI database. Its corresponding amino acid sequence included the putative conserved domains of Peptidase_S8, subtilase family and AprE, subtilisin-like serine proteases. Rapid amplification of 5’ and 3’ cDNA ends (RACE) was used to generate the full length (1385 bp) gene, with a 429 bp open reading frame. The gene encompasses coding regions for a catalytic triad formed by Asp-74, His-100 and Ser-110.

【 授权许可】

Unknown