Epitopes associated with mature spores not recognized on Kudoa thyrsites from recently infected Atlantic salmon smolts

Diseases of Aquatic Organisms

Catherine A. Young Simon R. M. Jones¹ 

关键词: Kudoa thyrsites;    Myxosporea;    Salmo salar;    In situ hybridization;    Immunohistochemistry;   

DOI  :  10.3354/dao063267

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Atlantic salmon Salmo salar skeletal muscle was examined for Kudoa thyrsites by polymerase chain reaction (PCR) and positive fish were further examined by in situ hybridization (ISH) and immunohistochemistry (IHC). Theinfection was detected in 42% of salmon by PCR following a 60 d exposure to infective seawater at a temperature of 10°C (= 600 degree-days, °D). The parasite was detected by ISH in skeletal and cardiac muscle but not in gill, kidney, spleen,liver, stomach, intestine, pyloric caeca and skin. None of 4 monoclonal antibodies (2F4, 4H2, 1H2, 3E8) raised against mature K. thyrsites spores reacted with the stages identified by ISH following a 600 °D exposure, but they did react withISH-identified stages following a 1600 °D exposure. In contrast, a polyclonal antibody reacted with K. thyrsites stages in salmon with both 600 and 1600 °D exposures, suggesting that the parasite observed in 600 °D infections representsan antigenically distinct developmental stage of K. thyrsites.

【 授权许可】

Unknown