Nutrient starvation affects expression of LC3 family at the feto-maternalinterface during murine placentation

Journal of Veterinary Medical Science

Masato HIYAMA²  Nobue KUNIYOSHI²  Sinji SUGI²  Hiroyuki IMAI¹  Yasuo KISO²  Ken Takeshi KUSAKABE²  Kiyoshi KANO²  Ai TAKESHITA³ 

[1] Laboratory of Veterinary Anatomy, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753�?8515, Japan;Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753�?8515, Japan;Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Science, Osaka Prefecture University, Osaka 598�?8531, Japan

关键词: autophagy;    LC3;    mTOR;    placenta;    starvation;   

DOI  :  10.1292/jvms.14-0490

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(39)LC3 �? the mammalian homolog of Atg8 �? was found as autophagosome membrane binding proteinin mammals and widely used as an autophagosomal marker. LC3A, B and C show differentexpression patterns in each tissue. The aim of this study was to reveal the differences ofexpression patterns among LC3 families in mouse placenta under normal condition andnutrient starving condition. LC3A and B were highly expressed in decidual cells. LC3A andB were increased in D14 compared with D12 and D16 in mouse placenta, while LC3C wasdecreased. Starvation induced increase in LC3B expression specifically.Immunohistochemistry showed different expression patterns among LC3A, B and C. LC3Aexpression in syncytiotrophoblast was vanished by starvation. The results of real timeRT-PCR suggested differences between D12 and D16 in autophagic cascade induced bystarvation. Taken together, this study suggests that autophagy could play a role inplacental invasion system and that nutrient starvation affects LC3B expression.

【 授权许可】

Unknown