Effect of Pomphorhynchus laevis (Acanthocephala) on putative neuromodulators in the intestineof naturally infected Salmo trutta

Diseases of Aquatic Organisms

Flavio Pironi¹  Bahram Sayyaf Dezfuli¹  Luisa Giari¹  Cinzia Domeneghini¹  Giampaolo Bosi¹ 

关键词: Acanthocephalan infection;    Immunohistochemical;    Neuromodulators;    Digestive tract;    Salmo trutta;   

DOI  :  10.3354/dao051027

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Immunohistochemical and pathological studies were carried out on the digestive tract of parasitized and uninfected specimens of Salmo trutta (L.). A total of 124 brown trout were collected on several occasions from 3 tributaries of theBrenta River, northern Italy. Twenty-eight individuals of S. trutta (22.6%) were parasitized with Pomphorhynchus laevis (Müller, 1776). The occurrence of P. laevis in the trout gut significantly increased the number of endocrine cellsimmunoreactive to calcitonin gene-related peptide (CGRP), β-endorphin, met-enkephalin, neuropeptide Y (NPY) and Substance P (SP) antisera. Moreover, bombesin-, cholecistokinin-8- (CCK-8), leu-enkephalin- and serotonin-(5-HT)-like immunoreactive cells were less numerous in the intestine of the parasitized brown trout. A strong positive immunoreactivity was observed in nerve fibres and neurones of the myenteric plexus of the parasitized fish; the antisera involved inthis positive reactivity were bombesin, met-enkephalin, SP and vasoactive intestinal peptide (VIP). More neurones immunoreactive to anti-CGRP and anti-5-HT sera were noted in the myenteric plexus and in the inner layer of the tunica muscularis of theinfected fish. Most of the above-mentioned neuromodulators are known to control gut motility, digestive/absorptive processes, as well as the immune response. The changes induced by parasites in the neuroendocrine system of the brown trout are discussed.

【 授权许可】

Unknown