Detection of Cryptosporidium parvum Oocysts in Environmental Water in Hokkaido, Japan

Journal of Veterinary Medical Science

Ikuo IGARASHI¹  Panagiotis KARANIS²  Takeshi MIKAMI¹  Kozo FUJISAKI¹  Takenori KAMADA¹  Eiji TAKAHASHI³  Xuenan XUAN¹  Yoshinori TSUSHIMA¹  Hideyuki NAGASAWA¹ 

[1] National Research Center for Protozoan Diseases, Obihiro University;Institute for Medical Parasitology, University of Bonn;Department of Veterinary Microbiology, Graduate School of Agricultural and Life Science, The University of Tokyo

关键词: cryptosporidiosis;    Cryptosporidium;    detection;    Hokkaido;    waterborne disease;   

DOI  :  10.1292/jvms.63.233

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(25)Cited-By(9)Control of cryptosporidiosis is important in public health. Rivers that are polluted with Cryptosporidium and drinking water that is treated for drinking water production from polluted rivers could result in the waterborne disease of cryptosporidiosis. We carried out an epidemiological study of natural water supplies in Hokkaido, one of the largest dairy prefectures in Japan. To detect Cryptosporidium oocysts in environmental water, the filtration method was used for 28 samples, which were collected from 10 rivers. A method adapted from the United States Environmental Protection Agency (U.S. EPA) filtration method using a cartridge filter has been used for the collection of samples. Oocysts were separated from a pellet by discontinuous sucrose gradient method. Twelve samples were collected from 10 rivers and parasites were purified by iron (III) flocculation method. Cryptosporidium parvum oocysts were identified with the immunofluorescence antibody technique using DIF kit (Cellabs Pty. Ltd., Sydney/Australia). We detected Cryptosporidium oocysts in 6 out of 10 rivers sampled. Fifty percentage (14/28) of the samples were Cryptosporidium-positive. The average number of Cryptosporidium oocysts was 16.73/100 L (max. 80 /100 L).

【 授权许可】

Unknown