Effects of high salinity, high temperature and pH on capsid structure of white spot syndrome virus

Diseases of Aquatic Organisms

Feng Yang¹  Heng Zhang¹  Weiyu Chen¹  Fang Li¹  Li Gu¹ 

关键词: WSSV;    Capsid;    Transmission electron microscopy;    Structural stability;   

DOI  :  10.3354/dao02511

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: The structural stability of white spot syndrome virus (WSSV) capsids at high salinity, high temperature and various pH values was studied. To obtain the viral capsids, the nucleocapsids were treated with high salinity. The results showed that high salinity treatment can cause the dissociation of VP15 and most of VP95 from the nucleocapsid, but there were no noticeable alterations in morphology and ultrastructure of the nucleocapsid and capsid. The capsids retained morphological integrity at temperatures <45°C but became aberrant at >60°C. In addition, the capsids were relatively resistant to strong acid conditions and were tolerant to a broad pH range of 1 to 10. However, morphological change occurred at pH 10.5. The capsids broke up into small pieces at pH 11 and completely degraded in 0.1 and 1.0 M NaOH. These results indicated that the WSSV capsid is acid-stable and alkali-labile.

【 授权许可】

Unknown