PCR testing for diagnosis of Ichthyophonus hoferi: Comment on Hamazaki et al. (2013)

Diseases of Aquatic Organisms

S. E. LaPatra¹  R. M. Kocan¹ 

关键词: Ichthyophonus hoferi;    Explant culture;    Polymerase chain reaction;    Diagnosis;    Confirmation;   

DOI  :  10.3354/dao02681

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: It is our opinion that Hamazaki et al. (2013; Dis Aquat Org 105:21�?25) overstate the usefulness of PCR as a field diagnostic technique and underestimate the accuracy and utility of in vitro explant culture. In order for field diagnostic studies to be meaningful they should accurately and dependably identify the infected individuals within a population, both subclinical and clinical cases. Although explant culture, like most techniques, can miss some infected individuals, ‘false positives�? are impossible, unlike for cPCR based methodologies.

【 授权许可】

Unknown