Canine semen's freeze with different concentrations of egg yolk and glycerol in tris and coconut wateer extenders

Ciência Rural

Silva, Lúcia Daniel Machado da¹  Cardoso, Rita de Cássia Soares¹  Silva, Alexandre Rodrigues¹ 

[1] FAVET/UECE, Fortaleza

关键词: semen;    dog;    freeze;    coconut water;    Tris;    glycerol;    egg yolk;   

DOI  :  10.1590/S0103-84782000000600017

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

The aim of the present research was to compare the efficiency of coconut water and Tris extenders on canine semen freezing. The semen from 5 German Shepherd dogs was collected by digital manipulation. The spermatic fraction of different dogs was evaluated about its macro and microscopic parameters and mixed in a pool. It was extended in coconut water or Tris, added of egg yolk (10 or 20%) and glycerol (4, 6 or 8%). Then, it was frozen by the method of NUNES et al. (1997) and thawed at 37oC after one week. A Tris extender superiority was shown at the conservation of vigor (2.3 ± 0.8), total (14.8 ± 5.1%) and secondary (14.4 ± 5.5%) spermatic defects in the protocols with 20% of egg yolk and 6% of glycerol. In all other protocols, there were no significant differences for the observed parameters. These results suggest a superiority of Tris extender over the coconut water extender on canine semen freeze, in the use of them added of 20% of egg yolk and 6% of glycerol.

【 授权许可】

CC BY-NC