Effect of temperature on defense parameters in Manila clam Ruditapes philippinarumchallenged with Vibrio tapetis

Diseases of Aquatic Organisms

Christine Paillard¹  Radouane Oubella¹  Bassem Allam¹ 

关键词: Immunomodulation;    Temperature;    Hemocyte;    Leucine aminopeptidase;    Lysozyme;    Disease;    Clam;    Vibrio;   

DOI  :  10.3354/dao059249

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Brown Ring Disease (BRD), a vibriosis affecting the clam Ruditapes philippinarum, is present on the Atlantic coasts of Western Europe and is considered to be a cold water disease. The present work investigated the effect of temperature onimmune response and its relationships with BRD development. Clams maintained at different temperatures (8, 14 and 21°C) were experimentally challenged with the pathogen Vibrio tapetis, the etiologic agent of BRD. Results demonstrated significanteffects of temperature on disease development and on hemolymph immune parameters including total and viable hemocyte counts, lysozyme and leucine aminopeptidase activities. Thirty days after challenge, clams maintained at 21°C displayed significantlyhigher values for all the measured immune parameters in comparison to specimens incubated at 14°C. Improved performance of the immune system was associated with a low BRD prevalence. The recovery process, which occured mainly at 21°C, was associated withhigh percentages of viable hemocytes and high activities of leucine amino-peptidase and lysozyme. This laboratory study clearly demonstrates that temperature strongly affects BRD development and clam immune response during infection. Favourable immunestatus at higher temperature may confer upon the clam a better capacity to fight the disease agent, and therefore to recover more easily.

【 授权许可】

Unknown