Direct ABC Immunohistochemistry to Encephalitozoon cuniculi

Journal of Veterinary Medical Science

Kenji OCHIAI¹  Jae-Hak PARK¹  Chitoshi ITAKURA¹ 

关键词: Encephalitozoon cuniculi;    immunohistochemistry;    rabbit;   

DOI  :  10.1292/jvms.55.325

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(14)Cited-By(1)Among the sera from 9 rabbits spontaneously infected with Encephalitozoon cuniculi, a serum which revealed a high titer for E. cuniculi by indirect protein A-gold (IPAG) immunohistochemistry and reacted with the outer layer of the shell of E. cuniculi spores on immunoelectron microscopical examination, was biotinylated. The biotinylated rabbit anti-E. cuniculi IgG reacted immunohistoche-mically with E. cuniculi, but not with other protozoa tested, namely Neospora caninum, Toxoplasma gondii and sarcocystis. The direct avidin-biotin-peroxidase complex (ABC) immunohistochemistry using biotinylated rabbit anti-E. cuniculi IgG in this study is a useful tool for the diagnosis and study of encephalitozoonosis.

【 授权许可】

Unknown