Portals of entry and systemic localization of proliferative gill disease organisms in channel catfish Ictalurus punctatus

Diseases of Aquatic Organisms

Adrien M. G. Belem¹  Linda M. Pote¹ 

关键词: Proliferative gill disease;    PGD ·Aurantiactinomyxon ictaluri;    Henneguya ictaluri n. sp.;    Myxozoa;    Actinosporean;    Myxosporean;    Polyclonal antibodies;   

DOI  :  10.3354/dao048037

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Immunized rabbit serum adsorbed in live catfish was used in indirect fluorescent antibody test (IFAT) to detect developmental life stages of Henneguya ictaluri n. sp. This myxozoan parasite is associated with proliferative gill disease inchannel catfish Ictalurus punctatus (Rafinesque) in the USA. Specific pathogen free fingerlings were experimentally infected with the actinosporean stage of H. ictaluri and necropsied 24, 48, 72, and 96 h post-infection. At 24 hpost-infection parasite stages were observed primarily in the gastric mucosa and submucosa but were also observed in the skin and buccal cavity. Ovoid organisms were detected in heart and blood vessels of the liver. From 48 to 72 h after exposure, fewerfluorescent organisms were located in all organs, with the exception of the gills, than were observed at 24 h. These organisms appeared to be degenerating except for those in the gills, which appeared to be multinucleated. By 96 h post-infection, theorganisms could not be detected in fish tissues with the exception of the stages in the gills, which appeared to be a preferred site of development. Throughout the entire 96 h period of study, no stage of the organism was detected in the brain. Infectedtissue sections treated with non-immune rabbit serum and non-infected tissue sections treated with immune rabbit sera all showed negative results by IFAT.

【 授权许可】

Unknown