Expression of CD14 and toll-like receptors 2 and 4 by milk neutrophils in bovine mammary glands infected with Corynebacterium bovis

Pesquisa Veterinária Brasileira

Batista, Camila F.²  Libera, Alice M.M.P. Della²  Blagitz, Maiara G.²  Azevedo, Luis Fernando F.²  Parra, Andrea C.²  Santos, Bruna P.²  Souza, Fernando N.¹ 

[1] Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;Universidade de São Paulo, São Paulo, Brazil

关键词: Immune response;    pattern recognition receptor;    mastitis;    milk;    polymorphonuclear neutrophil leukocyte;   

DOI  :  10.1590/S0100-736X2015000100001

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

This study evaluated the expression of CD14, toll-like receptor (TLR) 2 and TLR4 on the surface of milk neutrophils in bovine mammary glands infected with Corynebacterium bovis. Here, we used 23 culture-negative control quarters with no abnormal secretion on the strip cup test and milk somatic cell count lower than 1x105 cells/mL, and 14 C. bovis infected quarters. The identification of neutrophils, as well as, the percentage of neutrophils that expressed CD14, TLR2 and TLR4 were analyzed by flow cytometry using monoclonal antibodies. The present study encountered no significant difference in the percentages of milk neutrophils that expressed TLR2 and TLR4 or in the expression of TLR4 by milk neutrophils. Conversely, a lower median fluorescence intensity of TLR2 in milk neutrophils was observed in C. bovis-infected quarters. The percentage of neutrophils that expressed CD14 and the median fluorescence intensity of CD14 in milk neutrophils was also lower in C. bovis-infected quarters.

【 授权许可】

Unknown