Bone Regeneration by the Combined Use of Tetrapod-Shaped Calcium PhosphateGranules with Basic Fibroblast Growth Factor-Binding Ion Complex Gel in Canine SegmentalRadial Defects

Journal of Veterinary Medical Science

Shinsuke OHBA⁴  I-li LIU⁵  Nobuo SASAKI⁵  Ryohei NISHIMURA¹  Tetsushi TAGUCHI⁶  Sungjin CHOI⁴  Ung-il CHUNG³  Wataru KAMIMURA²  Hiroyuki KOYAMA²  Manabu MOCHIZUKI⁵  Muneki HONNAMI⁵  Nobuyuki SHIMOHATA⁴  Hironori HOJO⁴ 

[1] Laboratory of Veterinary Emergency Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1�?1�?1 Yayoi, Bunkyo-ku, Tokyo 113�?8657, Japan;Division of Tissue Engineering, Department of Vascular Regeneration, The University of Tokyo Hospital, 7�?3�?1 Hongo, Bunkyo-ku, Tokyo 113�?8655, Japan;Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7�?3�?1 Hongo, Bunkyo-ku, Tokyo 113�?8655, Japan;Division of Clinical Biotechnology, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7�?3�?1 Hongo, Bunkyo-ku, Tokyo 113�?8655, Japan;Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1�?1�?1 Yayoi, Bunkyo-ku, Tokyo 113�?8657, Japan;Smart Biomaterials Group, Biomaterials Unit, Nanoscale Materials Division, National Institute for Materials Science, 1�?1 Namiki, Tsukuba, Ibaraki 305�?0044, Japan

关键词: bone regeneration;    calcium phosphate;    collagen;    fibroblast growth factor;    histomorphometry;   

DOI  :  10.1292/jvms.14-0027

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Cited-By(1)The effect of tetrapod-shapedalpha tricalcium phosphate granules (Tetrabones® [TB]) in combination withbasic fibroblast growth factor (bFGF)-binding ion complex gel (f-IC gel) on bone defectrepair was examined. Bilateral segmental defects 20-mm long were created in the radius of5 dogs, stabilized with a plate and screws and implanted with 1 of the following: TB (TBgroup), TB and bFGF solution (TB/f group), and TB and f-IC gel (TB/f-IC group). Dogs wereeuthanized 4 weeks after surgery. Radiographs showed well-placed TB granules in thedefects and equal osseous callus formation in all the groups. Histomorphometry revealedthat the number of vessels and volume of new bone in the TB/f-IC group were significantlyhigher than those in the other groups. However, no significant differences inneovascularization and new bone formation were observed between the TB/f and TB groups.Furthermore, no significant difference in the lamellar bone volume or rate of mineralapposition was observed among groups. These results suggest that increased bone formationmight have been because of the promotion of neovascularization by the f-IC gel. Therefore,the combinatorial method may provide a suitable scaffold for bone regeneration in largesegmental long bone defects.

【 授权许可】

Unknown