In vitro MATURATION OF BOVINE OOCYTES WITH FSH+LH AND FSH IN CULTURE MEDIUM

Revista de Salud Animal

Hernández Pichardo, J.E¹  Fernández Reyes, F¹  Pichardo Cruz, A.I² 

[1] Universidad Autónoma Metropolitana-Xochimilco (UAM-X), México;Clínica privada, México

关键词: in vitro maturation;    oocytes;    ovine;    FSH;    L;   

DOI  :  

学科分类：兽医学

来源: Centro Nacional de Sanidad Agropecuaria

【 摘 要 】

For in vitro maturation of ovine oocytes, ovaries from slaughtered hybrid animals were obtained. Immediately after slaughter, ovaries were placed on physiological saline solution at 35oC. A total of 242 ovaries was collected and 1294 follicles with a size between 2 to 6 mm in diameter were obtained. From them, 107 oocytes showing 3 or more compact layers of cumulus cells and homogenous cytoplasm were selected and cultivated in a maturation medium TCM 199 supplemented with FSH and LH in concentrations of: 1) 5mg/mL of FSH and 5mg/mL of LH (22 oocytes). 2) 2.5 mg/mL of each FSH and LH (16 oocytes). 3) 5mg/mL of FSH (36 oocyres), 4) 2.5 mg/mL of FSH (33 oocytes). Subsequently, they were fixed and evaluated by the acetic orceine staining. The maturation results indicate that the group supplemented with FSH 2.5 mg/mL, showed the highest percentage (45.4%) of Metaphase II oocytes, followed by the group supplemented with 5 mg/mL of FSH (27.7%) and the group supplemented with 2.5 mg/mL of FSH + 2.5mg/mL LH (22.7%). Significant statistic differences among the groups were not observed (P< 0.05%). According to these results, it is concluded that the best medium for the ovine oocytes in vitro maturation is TCM-99 supplemented with FSH 2.5 mg/mL. The in vitro manipulation of ovine oocytes may allow the establishment of reproductive programs in the natural crossing age period and also out of it. Genetic material banks for several applications may also be established.

【 授权许可】

CC BY-NC