Analysis of L-type amino acid transporter in canine hepatocellularcarcinoma

Journal of Veterinary Medical Science

Ken ONDA²  Reiichiro SATO²  Hideharu OCHIAI¹  Kikumi OGIHARA³  Yuko NAYA³ 

[1]Research Institute of Biosciences, School of Veterinary Medicine, Azabu University, 1�?17�?71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252�?5201, Japan

[2]Laboratory of Internal Medicine 3, School of Veterinary Medicine, Azabu University, 1�?17�?71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252�?5201, Japan

[3]Laboratory of Pathology, School of Veterinary Medicine, Azabu University, 1�?17�?71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa 252�?5201, Japan

关键词: canine;    hepatoma;    LAT;    transporter;   

DOI  :  10.1292/jvms.14-0392

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(38)Analysis of L-type amino acid transport expression of hepatocellular carcinoma cells(HCCs) of the dog was performed. The leucine transport activity of canine HCCs was 0.628 ±0.018 nmol/mg protein/min. The inhibitor of LAT 2-aminobicyclo[2.2.1]heptane-2-carboxylicacid (BCH) reduced 90% of the activity at 1 mM. The deduced amino acid sequences of canineLAT2, LAT3 and LAT4 were well conserved in mammalians, exhibiting 89, 88 and 77% homology,respectively. RT-PCR revealed distinct LAT1 expression compared with normal hepatocytes.Western blotting analysis confirmed the potent LAT1 expression in canine HCCs but nothepatocytes, and real-time RT-PCR analysis indicated that canine HCCs possessed 28 timeshigher LAT1 expression than hepatocytes. These results indicated that the leucinetransport activity of canine HCCs was due to LAT1.

【 授权许可】

Unknown