Molecular Cloning of Canine RCAS1 cDNA

Journal of Veterinary Medical Science

Masaru OKUDA¹  Yasuhiko OKAMURA²  Zhiyong MA¹  Satoshi UNE²  Tanvir S. KHATLANI¹  Yasuho TAURA²  Munekazu NAKAICHI² 

[1] Department of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University;Department of Veterinary Surgery, Faculty of Agriculture, Yamaguchi University

关键词: canine;    cDNA;    RCAS1;   

DOI  :  10.1292/jvms.65.913

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Cited-By(2)Receptor-binding cancer antigen expressed on SiSo cells (RCAS1) is a novel cancer cell-surface antigen, strongly expressed in invasive cancers. RCAS1 inhibited the in vitro growth of immunocytes, and induced apoptotic cell death. The cloning of canine RCAS1 cDNA was carried out and identified from the mammary gland tumor of a dog. A canine RCAS1 cDNA of 864 bp in length has an open reading frame of 642 bp nucleotides encoding a protein of 213 deduced amino acids. The predicted amino acid sequence of canine RCAS1 showed 96.2% and 96.7% homologies with those of human and mouse RCAS1 respectively. Canine RCAS1 has an N-terminal transmembrane segment and a coiled-coil structure in the C-terminal protein, which are highly conserved in mouse and human RCAS1.

【 授权许可】

Unknown