Prevalence study of SNP c.421G>T in the ADAMTS2 gene responsible for dermatosparaxis in White Dorper sheep in Brazil

Pesquisa Veterinária Brasileira

Borges, Alexandre S.¹  Oliveira-Filho, José P.¹  Andrade, Danilo G.A.¹  Dalanezi, Felipe M.¹  Trecenti, Anelize S.¹  Cunha, Paulo Henrique J.² 

[1] Universidade Estadual Paulista, Botucatu, Brazil;Universidade Federal de Goiás, Goiânia, Brazil

关键词:  Cutaneous asthenia;    autosomal recessive;    PCR;    White Dorper;    sheep;   

DOI  :  10.1590/S0100-736X2016000200002

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

:Dermatosparaxis is an autosomal recessive disorder of connective tissue; the disorder is clinically characterized by skin fragility and hyperextensibility. Dermatosparaxis in White Dorper sheep is caused by a single nucleotide polymorphism (SNP) (c.421G>T) in the ADAM metalloproteinase with thrombospondin type 1 motif, 2 (ADAMTS2) gene. The aim of this study was to investigate the prevalence of this SNP in a White Dorper herd in São Paulo state, Brazil. In this study, we collected blood DNA samples from 303 White Dorper sheep and performed polymerase chain reaction to amplify the SNP region. The samples were sequenced to determine the presence of the SNP in the ADAMTS2 gene. The SNP prevalence in the studied population was 15.5%; this finding indicates that more effective control measures should be used to prevent the inheritance of SNP c.421G>T in the ADAMTS2 gene in Brazilian White Dorper herds.Index

【 授权许可】

Unknown