Immunogenic Properties of a Bovine Herpesvirus-1 (BHV-1) Recombinant Expressing Major Pseudorabies Virus (PrV) Glycoproteins in Combination

Journal of Veterinary Medical Science

Haruki OTSUKA³  Isao SHIBATA¹  Xuenan XUAN²  Yasunobu MATSUMOTO³  Yukinori IKEDA³ 

[1] Zen-noh Institute of Animal Health;The Research Center of Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine;Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Science, The University of Tokyo

关键词: Aujeszky's disease;    BHV-1;    glycoprotein;    PrV;    recombinant vaccine;   

DOI  :  10.1292/jvms.62.849

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(34)Cited-By(3)A recombinant bovine herpesvirus type 1 (BHV-1), designated BHV-1/ TF17-1, which expresses pseudorabies virus (PrV) glycoproteins gB, gC, gD, gE and gI in combination was constructed. To test the protective immunity, 10 mice were inoculated with BHV-1/TF17-1 and three weeks later 10 mice were intraperitoneally (i.p.) challenged with 20 LD50 virulent PrV (YS-81). BHV-1/TF17-1 protected all the mice from the PrV lethal challenge while all the control mice died in around 3 days. Mice vaccinated with BHV-1/TF17-1 acquired high PrV-neutralizing antibody titers and demonstrated strong delayed type hypersensitivity responses and moderate in vitro lymphocyte proliferative responses to PrV antigen. Since the major PrV glycoproteins were integrated into virions (probably into viral envelope), BHV-1/17-1 was neutralized with anti-PrV antiserum. However, the susceptibility of BHV-1/TF17-1 to anti-PrV antiserum is 2- to 4-fold lower than that of PrV vaccine lines. Our results demonstrated the possibility of BHV-1/17-1 as a vaccine to protect piglets from Audjesky's disease where maternal antibodies against PrV interfere attenuated live PrV vaccines.

【 授权许可】

Unknown