Monoclonal Antibodies Preventing Invasion of Neospora caninum Tachyzoites into Host Cells

Journal of Veterinary Medical Science

Kazunori IKE²  Akira ITO¹  Yuko UCHIDA²  Soichi IMAI²  Tetsuro KUROTAKI¹ 

[1] Research Center for Biologicals, The Kitasato Institute;Department of Parasitology, Nippon Veterinary and Animal Science University

关键词: invasion process;    monoclonal antibody;    Neospora caninum;    surface antigen;   

DOI  :  10.1292/jvms.66.1355

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(25)Cited-By(7)Twelve monoclonal antibodies (MAbs) against Neospora caninum tachyzoites were produced to specify the antigens related to the invasion of tachyzoites into host cells. In the assay to evaluate the inhibition activity, all these MAbs prevented the cultured Vero cells from the invading by the tachyzoites. These MAbs recognized approximately a 73 kDa antigen in Western blot analysis. Immunofluorescence assay and immune electron microscopy revealed that this 73 kDa antigen is a part of the surface antigens of N. caninum tachyzoite, and that the tachyzoite antigen identified plays an important role for invasion of host cells.

【 授权许可】

Unknown