Optimization of extracting solutions of antibacterial molecules from Cenchritis muricatus (Gastropoda:Littorinidae)

Revista Cubana de Medicina Tropical

Vázquez Perera, Antonio A¹  Otero González, Anselmo J²  Menéndez, Annia Alba¹  López Abarrategui, Carlos² 

[1] Instituto de Medicina Tropical Pedro Kourí (IPK), La Habana, Cuba;Centro de Estudio de Proteínas, La Habana, Cuba

关键词: Cenchritis muricatus;    antimicrobial molecules;    extracting solutions;   

DOI  :  

学科分类：传染病学

来源: SciELO

【 摘 要 】

Introduction: marine mollusks are natural reservoirs of molecules with therapeutic potential for the treatment of infectious diseases, at a time when many antibiotic-resistant strains are being described. Objective: to compare three solutions: 30% acetic acid, 50% methanol and saline-acid (NaCl 0.6 mol/L, 1% HCl) according to their capacities to extract molecules with antimicrobial activity from the marine mollusk Cenchritis muricatus. Methods: the three extraction solutions were used to process the biological material, and then, the obtained extracts were analyzed in terms of total protein concentration and the bacterial growth inhibition of Staphylococcus aureus and Escherichia coli strains by means of a turbidimetric bioassay using 96 well microplates in Luria-Bertani (LB) culture medium. Results: the highest total protein concentration (7.8 mg/mL) was found in the C. muricatus extract from the saline-acid solution. Additionally, 200 mg/mL of total proteins from the extract caused significant growth inhibition (p<0.001) of S. aureus (12.64 %) and E. coli (12.1 %) compared to the positive control of growth inhibition using chloramphenicol. Conclusions: according to these results, the saline-acid solution proved to be more efficient in extracting molecules with antibacterial activity that are likely to be antimicrobial peptides from C. muricatus.

【 授权许可】

CC BY-NC