Survival Change of Ventral Mesencephalon-Derived Progenitor Cells after Grafting into Unilateral Intact Adult Rat Striatum

Journal of Veterinary Medical Science

Jong Yoon BAHK⁴  Myeong Ok KIM¹  Moon Seok PARK¹  Hae Young LEE¹  Bong Chul CHUNG²  Phil-Ok KOH³  Shu Peng LI¹ 

[1] Division of Life Science, College of Natural Sciences and Applied Life Science (Brain Korea 21), Gyeongsang National University;Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology;Department of Anatomy, College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University;Department of Urology, College of Medicine, Gyeongsang National University

关键词: dopamine;    rat brain;    transplantation;    tyrosine hydroxylase;    ventral mesencephalon cells;   

DOI  :  10.1292/jvms.69.25

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(27)Neural transplantation is one of the most promising treatments for neurodegenerative disorders. Survival rates of embryonic dopamine (DA) neurons following transplantation are low, between 2% and 20% in a number of animal models. To further establish survival changes of the transplanted gestational day 13.5 ventral mesencephalic (VM) cells into left intact adult rat striata so that design strategies of increasing survival of DA neurons, the tyrosine hydroxylase (TH) expression of VM-derived progenitor cells has been examined using immunohistochemistry and Western blot analysis. TH immunostaining revealed that the grafted VM cells developed to mature TH-positive neurons strongly at 3 weeks, peaked at 4 weeks, thereafter, gradually dropped following the degenerative expression of the grafted cells at both 5 and 6 weeks after transplantation. Western blot analysis also showed that the TH proteins were maximally expressed at 4 weeks post-grafting. Our finding suggested that the peak of surviving VM-derived TH positive cells occurred approximately 4 weeks after transplantation.

【 授权许可】

Unknown