The Presence of a Short Form of p53 in Chicken Lymphoblastoid Cell Lines during Apoptosis

Journal of Veterinary Medical Science

Tamami TAKEDA³  Kazuhiko OHASHI²  Yuki ASADA³  Misao ONUMA²  Michihiro TAKAGI³  Chihiro SUGIMOTO¹ 

[1] Research Center for Zoonosis Control, Hokkaido University;Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University;Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University

关键词: apoptosis;    Marek's disease;    p53;   

DOI  :  10.1292/jvms.68.561

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(39)Cited-By(3)To examine the roles of a short form of p53 in the regulation of apoptosis in chicken lymphoblastoid tumor cell lines derived from Marek's disease (MD) and avian leukosis (AL), the expressions of the p53 proteins were analyzed in these cell lines in which apoptosis was chemically induced. In MSB1-O derived from MD, the expression of a 40 kDa protein of p53 was decreased and that of a 32 kDa protein, a short form of p53, was increased during apoptosis induced by actinomycin D. In 1104B1 derived from AL, the expressions of 42 and 32 kDa of p53 were increased during the apoptosis. The short form of p53 was undetectable in these cell lines when apoptosis was blocked by the pretreatment with endonuclease inhibitor, Zn2+, protease inhibitors, TPCK and TLCK, or caspase inhibitor, Z-VAD-FMK. In the transcriptional level, the expressions of bcl-2 and IAP were decreased in these cell lines during actinomycin D-induced apoptosis, but no change was detected in the expression level of p53. These results suggest that, in these chicken tumors, the short form of p53 could play a role in the initiation of apoptosis induced by the chemotherapeutic compound, and that the regulation of its expression may be important for the maintenance of transformation status.

【 授权许可】

Unknown