Chitosan and D-Glucosamine Induce Expression of Th1 Cytokine Genes in Porcine Spleen Cells

Journal of Veterinary Medical Science

Deog-Yong LEE¹  Jeong-Hee HAN²  Han-Sang YOO¹  In-Soo CHOI¹ 

[1] Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University;Department of Veterinary Medicine, Kangwon National University

关键词: chitosan;    D-glucosamine;    porcine cytokine expression;   

DOI  :  10.1292/jvms.64.645

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Cited-By(5)Chitosan, a polymer of D-glucosamine, is a polysaccharide derived from the chitin found in the exoskeleton of shellfish, such as shrimp or crabs. The effects of chitosan has been recognized that chitosan-fed farm animals demonstrated higher weight gains but less incidence of diseases than the unfed ones. However, these beneficial effects has not been elucidated clearly. In this study, we examined the modulatory effect of chitosan and D-glucosamine on the expression of porcine cytokines in vitro. Porcine spleen cells were cultured in the presence of chitosan and D-glucosamine, and the effects of chitosan on the cytokine mRNA expression were evaluated. Expressions of IL-2 and IFN-γ were increased in the chitosan-treated porcine spleen cells. Expressed cytokines in the D-glucosamine-treated cells were IL-2, IFN-γ, and IL-12 p40 subunit. In particular, IFN-γ was expressed more efficiently, and D-glucosamine was more effective for expressing the cytokine gene. These results suggest chitosan as well as D-glucosamine could induce the expression of cytokines as Th1 subset such as IL-2, IFN-γ.

【 授权许可】

Unknown