Isolation of Viable Cells in Canine Transmissible Sarcoma (CTS) Using Density Gradient Centrifugation

Journal of Veterinary Medical Science

Toru FUJINAGA¹  Saburo MINAMI¹  Yoshiharu OKAMOTO¹  Akira MATSUHASHI¹ 

关键词: canine transmissible sarcoma;    cell isolation;   

DOI  :  10.1292/jvms.58.11_1149

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(18)Cited-By(1)Density gradient centrifugation using Ficoll-Conray solution was attempted to isolate viable canine transmissible sarcoma (CTS) cells. The viability of isolated tumor cells increased from about 50% to >90%, and the yield of CTS cells was >50% with over 99% purity, in when an isolation solution density of 1.05 was used.

【 授权许可】

Unknown