Enzyme-Linked Immunosorbent Assay for Serological Survey of Equine Arteritis Virus in Racehorses

Journal of Veterinary Medical Science

Hiroshi IMAGAWA²  Takeo SUGIURA²  Kiichi SEKIGUCHI¹  Takashi KONDO²  Yoshio FUKUNAGA² 

[1] Department of Exotic Disease, National Institute of Animal Health;Epizootic Research Station, Equine Research Institute, The Japan Racing Association

关键词: ELISA;    equine arteritis virus;    serological survey;   

DOI  :  10.1292/jvms.60.1043

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)Cited-By(5)To examine antibodies against equine arteritis virus (EAV), an enzyme-linked immunosorbent assay (ELISA) using purified virus antigen was developed. The results of ELISA were compared with those of serum neutralization (SN) tests. The ELISA absorbance values and the SN titers in sera collected weekly from EAV-infected horses showed a similar pattern. The ELISA could detect antibody to EAV in horses experimentally infected with not only a homologous virus strain, which was used as the ELISA antigen, but also a heterologous strain. Using the ELISA, serum samples collected in 1996 from racehorses in three prefectures (Hokkaido, Ibaraki, and Shiga) were examined and there was no evidence of recent EAV infection among these racehorse populations in Japan. The ELISA should be a simple and highly specific method for rapid screening of EAV infection in racehorses.

【 授权许可】

Unknown