Neuroanatomical Distribution of Disease-Associated Prion Protein in Cases of Bovine Spongiform Encephalopathy Detected by Fallen Stock Surveillance in Japan

Journal of Veterinary Medical Science

Yuichi MATSUURA²  Morikazu IMAMURA²  Kei FUJII¹  Yoshihumi IWAMARU²  Kazuo KASAI²  Takashi YOKOYAMA²  Sadao ONOE¹  Kentaro MASUJIN²  Masuhiro TAKATA²  Yoshihisa SHIMIZU²  Shirou MOHRI²  Shigeo FUKUDA¹  Satoshi NIKAIDO¹  Hiroyuki OKADA² 

[1] Hokkaido Animal Research Center, Hokkaido Research Organization;Prion Disease Research Center, National Institute of Animal Health

关键词: BSE;    cattle;    immunohistochemistry;    prion;   

DOI  :  10.1292/jvms.11-0291

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(35)Cited-By(1)Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative disorder of cattle characterized by accumulation of the disease-associated prion protein (PrPSc) in the central nervous system (CNS). The immunohistochemical patterns and distribution of PrPSc were investigated in the CNS, brains, and spinal cords of 7 naturally occurring BSE cases confirmed by the fallen stock surveillance program in Japan. No animals showed characteristic clinical signs of the disease. Coronal slices of 14 different brain areas in each case were immunohistochemically analyzed using an anti-prion protein antibody. Immunolabeled PrPSc deposition was widely observed throughout each brain and spinal cord. Intense PrPSc deposition was greater in the thalamus, brainstem, and spinal cord of the gray matter than in the neocortices. The topographical distribution pattern and severity of PrPSc accumulation were mapped and plotted as immunohistochemical profiles of the different brain areas along the caudal-rostral axis of the brain. The distribution pattern and severity of the immunolabeled PrPSc in the CNS were almost the same among the 7 cases analyzed, suggesting that the naturally occurring cases in this study were at the preclinical stage of the disease. Immunohistochemical mapping of the PrPSc deposits will be used to clarify the different stages of BSE in cattle.

【 授权许可】

Unknown