期刊论文详细信息
Aquatic Microbial Ecology
Molecular identification of picoplankton populationsin contrasting waters of the Arabian Sea
Bernhard M. Fuchs1  Dagmar Woebken Mikhail V. Zubkov Peter Burkill Rudolf Amann1 
关键词: Phylogenetic composition;    Arabian Sea;    Oxygen minimum zone;    OMZ;    Fluorescence in situ hybridisation;    Catalyzed reporter deposition (CARD)-FISH;    Oligonucleotide probes;    Flowcytometry;   
DOI  :  10.3354/ame039145
学科分类:生物科学(综合)
来源: Inter-Research
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【 摘 要 】

ABSTRACT: The composition of picoplankton in the southern oligotrophic, northern mesotrophic waters and deep oxygen minimum zone (OMZ) of the Arabian Sea was determined by 16S ribosomal RNA gene cloning and fluorescence in situ hybridisation(FISH). It was hypothesised that the composition of the heterotrophic picoplankton would be different in these contrasting waters. To reduce the total diversity, cells were sorted by flow cytometry according to their scatter and DNA content before PCRamplification. The 16S rRNA clone libraries resulting from flow-sorted populations were different and often dominated by a small number of clades. Libraries from the Prochlorococcus-dominated southerly waters were dominated by sequences related touncultured clusters of SAR11, SAR86 and Actinobacteria (HGC I). From surface waters of the Synechococcus-dominated northern part of the Arabian Sea, mostly sequences related to the uncultured gammaproteobacterial group ‘Svalbard’ and HGCI were retrieved. The clone libraries from the OMZ were also dominated by sequences falling in the clades SAR11 and SAR406, but included sequences related to those of sulfate-reducing (Desulfosarcina, Desulfofrigus) and sulfide-oxidisingbacteria (endosymbionts of Riftia and Calyptogena). With a recently developed more sensitive FISH protocol approximately 60% of all DAPI stained cells could be identified by general probes as Bacteria, Cren- or Euryarchaeota in bothprovinces of the Arabian Sea; 40% remained undetected. On this level and on that of the major phylogenetic groups like Alpha- and Gammaproteobacteria only minor differences were detected by FISH. However, the composition of heterotrophic picoplanktonclearly differed for the proteobacterial subgroups SAR86, SAR11 and SAR116. These were more abundant in the oligotrophic waters throughout the water column than in the mesotrophic surface waters and the OMZ. This supports our original hypothesis that thecontrasting waters in the Arabian Sea harbor different heterotrophic picoplankton communities. In the future, FISH with a larger set of probes for more narrow phylogenetic groups will enable us to quantify these differences in more detail.

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