Technological Advances in Bifidobacterial Molecular Genetics: Application to Functional Genomics and Medical Treatments

Bioscience of Microbiota, Food and Health

Mikiyasu SAKANAKA²  Atsushi YOKOTA²  Yosuke HIRAYAMA²  Yasunobu KANO¹  Satoru FUKIYA² 

[1] Department of Molecular Genetics, Kyoto Pharmaceutical University;Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

关键词: Bifidobacterium;    molecular genetics;    cryptic plasmid;    heterologous gene expression;    targeted gene inactivation;    functional genomics;    enzyme/prodrug therapy;   

DOI  :  10.12938/bmfh.31.15

学科分类：生物科学（综合）

来源: Nihon Bifizusukin Senta / Japan Bifidus Foundation

【 摘 要 】

References(56)Cited-By(3)Bifidobacteria are well known as beneficial intestinal bacteria that exert health-promoting effects in humans. In addition to physiological and immunological investigations, molecular genetic technologies have been developed and have recently started to be applied to clarify the molecular bases of host-Bifidobacterium interactions. These technologies include transformation technologies and Escherichia coli-Bifidobacterium shuttle vectors that enable heterologous gene expression. In this context, a plasmid artificial modification method that protects the introduced plasmid from the restriction system in host bifidobacteria has recently been developed to increase transformation efficiency. On the other hand, targeted gene inactivation systems, which are vital for functional genomics, seemed far from being practically applicable in bifidobacteria. However, remarkable progress in this technology has recently been achieved, enabling functional genomics in bifidobacteria. Integrated use of these molecular genetic technologies with omics-based analyses will surely boost characterization of the molecular basis underlying beneficial effects of bifidobacteria. Applications of recombinant bifidobacteria to medical treatments have also progressed.

【 授权许可】

Unknown