Effects of Polyethylene Glycol Administration and Bone Marrow Stromal CellTransplantation Therapy in Spinal Cord Injury Mice

Journal of Veterinary Medical Science

Hiroshi NAKAZAWA¹  Tomoya HARAGUCHI²  Kenji TANI¹  Yasutaka ODA¹  Kazuhito ITAMOTO²  Atsunobu ISOZAKI¹  Yasuho TAURA¹ 

[1] Department of Veterinary Surgery, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677�?1 Yoshida, Yamaguchi 753�?8515, Japan;Division of Veterinary Surgery, Animal Medical Center, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677�?1 Yoshida, Yamaguchi 753�?8515, Japan

关键词: bone marrow stromal cell;    polyethylene glycol;    spinal cord injury;    synergistic effects;   

DOI  :  10.1292/jvms.13-0167

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(33)Cited-By(1)Bone marrow stromal cell (BMSC) transplantation has been reported as treatmentsthat promote functional recovery after spinal cord injury (SCI) in humans and animals.Polyethylene glycol (PEG) has been also reported as treatments that promote functionalrecovery after spinal cord injury (SCI) in humans and animals. Therefore, administrationof PEG combined with BMSC transplantation may improve outcomes compared with BMSCtransplantation only in SCI model mice. SCI mice were divided into a control-group,BMSC-group, PEG-group and BMSC+PEG-group. BMSC transplantation and PEG administration wereperformed immediately after surgery. Compared to the control-group, PEG- andBMSC+PEG-groups showed significant locomotor functional recovery 4 weeks after therapy. Weobserved no significant differences among the groups. In the BMSC- and BMSC+PEG-groups,immunohistochemistry showed that many neuronal cells aggressively migrated toward theglial scar from the region rostral of the lesion site. In the control- and PEG-groups, theboundary of the injured regions was covered with astrocytes, and a few neuronal cells weremigrated toward the glial scar. We conclude that combined BMSC transplantation with PEGtreatment showed no synergistic effects on locomotor functional recovery or beneficialcellular events. Further studies may improve the effect of the treatment, includingmodification of the timing of BMSC transplantation.

【 授权许可】

Unknown