Stable isotope marking of otoliths during vaccination: a novel method for mass-marking fish

Aquaculture Environment Interactions

Tom Hansen¹  Fletcher Warren-Myers¹  Stephen E. Swearer¹  Arne J. Jensen¹  Per Gunnar Fjelldal¹  Tim Dempster¹ 

关键词: Atlantic salmon;    Barium;    Escape;    Salmo salar;    Strontium;   

DOI  :  10.3354/aei00103

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Tagging or marking of fishes enables the collection of population-based information for ecological research, yet few techniques enable 100% mark detection success. We tested a new mass-marking technique: otolith marking with enriched stable isotopes delivered during vaccination. Atlantic salmon (Salmo salar) parr were injected in either the abdominal cavity or muscle with a combination of enriched ¹³⁷Ba, ⁸⁶Sr and ²⁶Mg, using 1 of 3 carrier solutions (water, vaccine, vaccine mimic). Laser ablation inductively coupled plasma mass spectrometry of the otoliths indicated that ¹³⁷Ba and ⁸⁶Sr isotope enrichment treatments achieved 100% mark success, with 0 to 34% success for ²⁶Mg, compared to experimental controls. Mark strength was greater when enriched isotopes were injected into the abdominal cavity compared to muscle. Isotope markers did not affect fish condition or survival. Marks could be differentiated with 100% success from the background levels present in wild parr collected from 22 Norwegian rivers. Stable isotope marking via vaccination with enriched stable isotopes is a mass-marking technique that, once optimised, could allow for cost-effective differentiation of wild and escaped farmed fish for each independent farming area.

【 授权许可】

Unknown