Rapid and Direct Detection of Clostridium chauvoei by PCR of the 16S-23S rDNA Spacer Region and Partial 23S rDNA Sequences

Journal of Veterinary Medical Science

Kinya YAMAMOTO²  Mari NORIMATSU¹  Yoshimasa SASAKI²  Yutaka TAMURA²  Akemi KOJIMA²  Yukie TETSUKA³ 

[1] Institute for Animal Health;National Veterinary Assay Laboratory;Tochigi Pre. Kenhoku Animal Hygiene Center

关键词: Clostridium chauvoei;    PCR;    16S-23S rDNA spacer region;   

DOI  :  10.1292/jvms.62.1275

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Cited-By(5)Clostridium chauvoei causes blackleg, which is difficult to distinguish from the causative clostridia of malignant edema. Therefore, a single-step PCR system was developed for specific detection of C. chauvoei DNA using primers derived from the 16S-23S rDNA spacer region and partial 23S rDNA sequences. The specificity of the single-step PCR system was demonstrated by testing 37 strains of clostridia and 3 strains of other genera. A 509 bp PCR product, which is a C. chauvoei-specific PCR product, could be amplified from all of the C. chauvoei strains tested, but not from the other strains. Moreover, this single-step PCR system specifically detected C. chauvoei DNA in samples of muscle from mice 24 hr after inoculation with 100 spores of C. chauvoei, and in clinical materials from a cow affected with blackleg. These results suggest that our single-step PCR system may be useful for direct detection of C. chauvoei in culture and in clinical materials from animals affected with blackleg.

【 授权许可】

Unknown