Immunohistochemical characterization of bovine brain lesions caused by Senecio sp

Pesquisa Veterinária Brasileira

Fredo, Gabriela¹  Driemeier, David¹  Wouters, Flademir¹  Boabaid, Fabiana M.¹  Wouters, Angelica T.B.¹ 

[1] Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil

关键词: Poisonous plants;    Senecio spp.;    hepatic encephalopathy;    status spongiosus;    GFAP;    S100;    vimentin;    plant poisoning;    cattle.;   

DOI  :  10.1590/S0100-736X2013001100007

来源: Colegio Brasileiro de Patologia Animal-CBPA

【 摘 要 】

Senecio spp. poisoning is an important cause of illness and death of cattle in Rio Grande do Sul, Brazil, and is often manifested by neurologic clinical signs and histological brain changes. Histological evaluation was performed on liver and brain samples of ten cattle naturally poisoned by Senecio sp. Samples of cerebrum, brainstem and cerebellum were stained with periodic acid-Schiff (PAS), and immunohistochemistry was carried out, employing anti-fibrillary acidic protein (GFAP), anti-S100 protein and anti-vimentin antibodies. The histological finding in the brain included mild to severe vacuolation in the white matter and the junction of gray and white matter, characterized as spongy degeneration. Histochemical and immunohistochemical staining revealed no significant findings compared with the brains of eleven adult cattle without liver and/or brain changes used as controls. Index

【 授权许可】

CC BY-NC