Concentration of carp edema virus (CEV) DNA in koi tissues affected by koi sleepy disease (KSD)

Diseases of Aquatic Organisms

John Hellmann¹  Mikolaj Adamek¹  Sven Michael Bergmann¹  Dieter Steinhagen¹  Felix Teitge¹  Dirk Willem Kleingeld¹  David Michael Stone¹  Verena Jung-Schroers¹  Keith Way¹  Martin Runge¹ 

关键词: Carp edema virus;    Koi sleepy disease;    Real-time PCR;    Common carp;    Koi Cyprinus carpio;   

DOI  :  10.3354/dao02994

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Carp edema virus (CEV), the causative agent of ‘koi sleepy disease�? (KSD), appears to be spreading worldwide and to be responsible for losses in koi, ornamental varieties of the common carp Cyprinus carpio. Clinical signs of KSD include lethargic behaviour, swollen gills, sunken eyes and skin alterations and can easily be mistaken for other diseases, such as infection with cyprinid herpesvirus 3 (CyHV-3). To improve the future diagnosis of CEV infection and to provide a tool to better explore the relationship between viral load and clinical disease, we developed a specific quantitative PCR (qPCR) for strains of the virus known to infect koi carp. In samples from several clinically affected koi, CEV-specific DNA was present in a range from 1 to 2046000 copies, with a mean of 129982 copies and a median of 45 copies per 250 ng of isolated DNA, but virus DNA could not be detected in all clinically affected koi. A comparison of the newly developed qPCR, which is based on a dual-labelled probe, to an existing end-point PCR procedure revealed higher specificity and sensitivity of the qPCR and demonstrated that the new protocol could improve CEV detection in koi. In addition to improved diagnosis, the newly developed qPCR test would be a useful research tool. For example, studies on the pathobiology of CEV could employ controlled infection experiments in which the development of clinical signs could be examined in parallel with a quantitative determination of virus load.

【 授权许可】

Unknown