Adrenal Steroids in Serum during Danazol Therapy, Taking into Account Cross-Reactions between Danazol Metabolites and Serum Androgens

Endocrine Journal

KATSUMI ARAKI¹  TOSHINOBU NAKAGAWA¹  GEN YAMASHIRO¹  KAZUTOMO AKASOFU¹  KOUICHI MURAKAMI¹ 

[1] Department of Obstetrics and Gynecology, Kanazawa University

关键词: Danazol;    Adrenal androgens;    Endometriosis;   

DOI  :  10.1507/endocrj.40.659

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(17)Cited-By(9)To investigate the changes in the serum androgen concentrations and the Free Androgen Index (FAT) in women during danazol therapy, we measured the serum concentrations of adrenal steroids and danazol metabolites, and then examined the effects of danazol metabolites on assays for serum androgens. Thirteen women who had endometriosis were treated with danazol (300 or 400 mg/day) for 8 to 16 weeks. Blood samples were taken before, during, and after the medication. During the danazol therapy, serum testosterone (T), cortisol (F), and sex-hormone binding globulin (SHBG) significantly decreased (P<0.05); but serum dehydroepiandrosterone-sulfate (DHEAS) and FAI increased (P<0.05). The serum concentrations of danazol metabolites were: danazol, 209.0±28.3 (ng/mL, mean±SEM); 1-2-hydroxymethyl ethisterone, 114.4±8.4; and 2-hydroxymethyl ethisterone, 660.0±54.2. There was considerable cross-reaction between danazol metabolites and androgens [T, androstenedione (A), and dehydroepiandrosterone (DHEA)] in the direct assays. As for the ratios of adrenal steroids in serum, the DHEAS/F, DHEAS/DHEA, and 11-deoxycortisol (S)/F ratios increased (P<0.05). We conclude that the increase in FAT and DHEAS represents increased native androgenic activity with danazol, and the changes in adrenal steroid ratios in serum indicate the inhibition of 11β-hydroxylase and sulfatase activities during danazol therapy.

【 授权许可】

Unknown