Lacaziosis-like disease in Tursiops truncatus from Brazil: a histopathological and immunohistochemical approach

Diseases of Aquatic Organisms

Angélica María Sánchez-Sarmiento¹  Fernando Esperón¹  Pedro Castilho¹  Samira Costa-Silva¹  Juliana Marigo¹  Kátia R. Groch¹  Carlos Sacristán¹  Larissa Rosa De Oliveira¹  Rodrigo Albergaria Réssio¹  Cristiane K. M. Kolesnikovas¹  Fábio Gonçalves Daura-Jorge¹  Paulo Henrique Ott¹  Natália Coelho Couto De Azevedo Fernandes¹  Paulo C. Simões-Lopes¹  José Luiz Catão-Dias¹ 

关键词: Lobomycosis;    Paracoccidioides brasiliensis;    Cetacean;    Yeast;    Immunohistochemistry;    Tursiops;    Bottlenose dolphin;   

DOI  :  10.3354/dao02954

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Cetacean lacaziosis-like disease or lobomycosis-like disease (LLD) is a chronic skin condition caused by a non-cultivable yeast of the order Onygenales, which also includes Lacazia loboi, as well as Paracoccidioides brasiliensis and P. lutzii, respectively responsible for lacaziosis and paracoccidioidomycosis in humans. Complete identification and phylogenetic classification of the LLD etiological agent still needs to be elucidated, but preliminary phylogenetic analyses have shown a closer relationship of the LLD agent to Paracoccidioides spp. than to L. loboi. Cases of LLD in South American cetaceans based on photographic identification have been reported; however, to date, only 3 histologically confirmed cases of LLD have been described. We evaluated multiple tissue samples from 4 Tursiops truncatus stranded in the states of Santa Catarina (n = 3) and Rio Grande do Sul (n = 1), southern Brazil. Macroscopically, all animals presented lesions consistent with LLD. Hematoxylin-eosin, periodic acid-Schiff, Grocott’s methenamine silver, and Mayer’s mucicarmin stains were used for histological evaluation. Microscopically, numerous refractile yeasts (4-9 µm in diameter) were observed in skin samples (4/4), and for the first time in dolphins, also in a skeletal muscle abscess (1/4). Immunohistochemistry using anti-P. brasiliensis glycoprotein gp43 as a primary antibody, which is known to cross-react with L. loboi and the LLD agent, was performed and results were positive in all 4 cases. We describe 3 new cases of LLD in cetaceans based on histopathology and immunohistochemistry. This is the first report of LLD in the muscle of cetaceans.

【 授权许可】

Unknown